| NBRP Rat No | 0469 |
|---|---|
| 品系名稱 | W-Tg(Gnrh1-EGFP)Nphy |
| 俗名 | Wistar-Tg(Gnrh1-EGFP)Nphy, Wistar-Tg(GnRH-EGFP)Nphy |
| 毛色 | albino (c) |
| 近交系世代 | F?+13 (April 2012) |
| 使用限制 | 使用者在發表研究成果時，應引用提供者指定的文獻。 |
| Genetic Status | Transgene |
| 應用 | 神經生理學、神經內分泌學 |
| Gene Affected | GFP: Green Fluorescent Protein, jellyfish |
| 來源 | 2001年，利用上述基因製作了基因轉殖大鼠，並建立該品系。使用Crlj:WI（從日本Charles River購入的Wistar大鼠）產生Tg大鼠。此後，持續透過與Jla:Wistar（日本醫學動物資材研究所）交配來維持。 |
| 特徵 | 基因改造生物。在大鼠性腺刺激素釋放激素（Gonadotropin Releasing Hormone, GnRH）promoter控制下，表達綠色螢光蛋白（EGFP）。 在免疫陽性的GnRH神經元中，有30-40%顯示出強的EGFP螢光。 |
| 繁殖狀態 | 隨著近親繁殖，生育力可能會下降。因此，有必要定期與wild品系進行交配。 |
Reference 參考文獻
- Kato M, Ui-Tei K, Watanabe M, Sakuma Y. Characterization of voltage-gated calcium currents in gonadotropin-releasing hormone neurons tagged with green fluorescent protein in rats. Endocrinology. 144(11):5118-25, 2003.
- Yin C., Ishii H., Tanaka N., Sakuma Y., Kato M. Activation of A-type g-amino butyric acid receptors (GABAARs) excites gonadotropin-releasing hormone (GnRH) neurones isolated from adult rats. J Neuroendocrinol. 2008 May;20(5):566-75.
- Kato M, Tanaka N, Usui S, Sakuma Y. The SK channel blocker apamin inhibits slow afterhyperpolarization currents in rat gonadotropin-releasing hormone neurones. J Physiol. 15;574(Pt 2):431-42, 2006.
- Watanabe M., Sakuma Y., Kato M. GABAA receptors mediated excitation in adult rat GnRH neurons. Biol Reprod. 2009 Aug;81(2):327-332.
- Kato M., Tanaka N., Ishii H., Yin C., Sakuma Y. Ca2+ channels and Ca2+-activated K+ channels in adult rat gonadotrophin-releasing hormone neurones. J Neuroendocrinol. 2009 Mar;21(4):312-315.
- Tanaka N., Ishii H., Yin C., Koyama M., Sakuma Y., Kato M. Voltage-gated Ca2+ channel mRNAs and T-type Ca2+ currents in rat gonadotropin-releasing hormone neurons. J. Physiol. Sci. 2010 May;60(3):195-204.
- Koyama M, Yin C, Ishii H, Sakuma Y, Kato M. Somatostatin inhibition of GnRH neuronal activity and the morphological relationship between GnRH and somatostatin neurons in rats. Endocrinology. 2012 Feb;153(2):806-814.
- Tada H, Kuroki Y, Funabashi T, Kamiya Y, Goto T, Suyama K, Sano A, Mitsushima D, Etgen AM, Takahashi T. Phasic synaptic incorporation of GluR2-lacking AMPA receptors at gonadotropin-releasing hormone neurons is involved in the generation of the luteinizing hormone surge in female rats. Neuroscience. 2013 Sep 17;248:664-66
