- Response Time: <5 sec
- Lowest Detection Limit: 1 nM
- Sensitivity: 2 pA/nM
- Application: Cell Cultures
The image (right) shows an abdominal x-ray of an apparatus consisting of two customized ISO-NOP nitric oxide probes, 4-channel pH catheter and Teflon nasogastic tube. (Courtesy Prof. K.E.L. McColl, University Department of Medicine and Therapeutics, Western Infirmary, Glasgow, Scotland., lijima, K., et al. Gastroenterology 2003:122:1248-1257)
The original nitric oxide probe
Ideal for cell cultures, cell suspensions and many other applications.The ISO-NOP is a popular, robust and high performance sensor encased within a 2 mm diameter disposable stainless steel protective sleeve. The tip of the sleeve is covered with a NO-selective membrane. Replacement membrane sleeves can be purchased separately (WPI #5436) and require an internal electrolyte (WPI #7325).
NO-3 and NO-2 Detection
A simple change in experimental protocol will enable the ISO-NOP to be conveniently used for rapid accurate determination of nitrite (NO-2) and nitrate (NO-3) concentration in samples. Using this method a detection limit for NO-2 or NO-3 as low as 1nM is routinely possible.
Selectivity of WPI's NO sensors
The ideal NO sensor should be insensitive to other reactive species likely to be present within the measurement environment. Conventional Nafion coated carbon fiber NO sensor exhibits a large response to such species. WPI’s unique NO sensor technology utilizes an novel surface membrane which amplifies the response to NO whilst eliminating responses to a vast range of reactive species, including nitrite, absorbic acid, hydrogen peroxide, catecolamines, and much more.
| Outside Diameter | 2mm |
| Response Time | <5 sec |
| Lowest Detection Limit/Range | 1nM |
| Upper Detection Limit/Range | 80uM (beyond 80uM, sensor accuracy is not guaranteed) |
| Nominal Sensitivity (New sensor) | ≤2pA/nM |
| Interferent (Selectivity Coefficient) | NaNO2 (10-6 or better) |
| Drift | <1pA/min. |
| Poise Voltage | 865mV |
| Typical Quiescent Baseline Current, 25°C | 3,000pA |
| Acceptable Baseline Range | 1,000-8,000pA |
| Polarization Time | 12+ hours |
| Recommended Polarization Solution | 0.1M KI/H2SO4 |
| Physiological Interference | None |
| 5399 | T-Adapter Kit (pkg. of 3) |
| 5435 | ISO-NOP Startup Kit |
| 5436 | Replacement Sleeves (pkg. of 4) |
| 7325 | ISO-NO Electrolyte Filling Solution (10mL) |
| 7357 | Nitrite Standard Solution, 1g/L (100mL) |
| 7363-4 | Enzol – Enzymatic detergent (1 gal.) |
| 7364 | Cidex |
| 7521 | ISONO Electrolyte, CO2 insensitive Filling Solution (10mL) |
| JUV | ISO-NOP Rejuvenator |
| MF28G67-5 | MicroFil electrolyte filling needle (pkg. of 5) |
| NSA-3 | Pre-Polarizer – 3 channel, ISO-NO Activator |
| Outside Diameter | 2mm |
