ApoE knockout rats
HsdSage:SD-ApoEem1Sage
- 給予ApoE KO大鼠高脂飲食會顯著縮短其壽命（Inotiv不建議給ApoE KO大鼠餵食高脂飲食）
- 在早期（5週齡和10週齡）時，ApoE KO大鼠顯示顯著更高的血清膽固醇
- 背景品系：Sprague Dawley
- 同合子剔除，經Western blot顯示ApoE蛋白完全缺失
- 接合性基因型 | 同合子
Apolipoprotein E（ApoE）是乳糜微粒(chylomicron)中的一個關鍵缺輔基蛋白(apoprotein)，它能與肝細胞和週邊細胞上的特定受體結合。ApoE的缺陷會導致脂蛋白、脂溶性維生素和膽固醇進入淋巴系統後再進入血液的運輸受阻。
ApoE 對於脂質的正常代謝至關重要。它在肝臟、腸道和大腦中表達，防止血漿中富含膽固醇的顆粒積聚。它因其在心血管疾病和脂蛋白運輸中的作用而被廣泛研究；ApoE近期被認為與阿茲海默症和認知有關，使其成為研究動脈粥狀硬化、阿茲海默症和神經損傷的有用模型。
來源
ApoE KO 大鼠模型最初是在密蘇里州聖路易斯的 SAGE Labs, Inc. 創建的，並在賓夕法尼亞州Boyertown工廠進行分發。該生產線透過 Envigo 收購的SAGE Labs 原有的動物庫存而繼續維持，接著 Envigo 於 2021 年被 Inotiv 收購。
- 16 bp deletion within Exon 3 on chromosome 1
- Administration of high fat diets to ApoE KO rats has resulted in significantly reduced lifespan (Inotiv does not recommend administering high fat diets to ApoE KO rats)
- At an early age (5- and 10-week-old), ApoE knockouts demonstrate significantly higher serum cholesterol
- Background strain: Sprague Dawley
- Homozygous knockouts exhibit complete loss of ApoE protein via Western blot
- Zygosity genotype | Homozygous as live colony
Apolipoprotein E (ApoE) is a critical apoprotein of the chylomicron which binds to a specific receptor on liver cells and peripheral cells. Defects in ApoE result in disrupted transportation of lipoproteins, fat-soluble vitamins and cholesterol into the lymph systems, and then into blood.
ApoE is essential for the normal metabolism of lipids. It is expressed in the liver, intestines and brain, preventing the accumulation of cholesterol-rich particles in plasma. Widely studied for its role in cardiovascular disease and lipoprotein transport, it has more recently been implicated in Alzheimer’s disease and cognition, making this a useful model for the study of atherosclerosis, Alzheimer’s and nerve injury.
Origin
The ApoE KO rat model was originally created at SAGE Labs, Inc. in St. Louis, MO and distributed out of the Boyertown, PA facility. The line continues to be maintained through the original SAGE Labs animal inventory acquired by Envigo, then Envigo was acquired by Inotiv in 2021.
