SYS-773 Duo 773 靜電計
- 2-channel intracellular amplifier
- For intracellular dual or differential studies
- Built-in active filtering
- Comes complete with 2 probe headstages, normally one high impedance and one low impedance
- Opti
Headstage
For intracellular dual or differential studies, the Duo773 has separate negative capacity controls and built-in active filtering that allows the precise balancing of time constants for artifact-free differential measurement. Comes complete with two probe headstages, 10^15 Ohms & 10^11 Ohms probes to monitor signals from ion-specific micro-electrodes as well as KCl-filled electrodes. Two gold-plated, epoxy sealed miniature active probes can be positioned directly to the measurement site. Microelectrode holders containing an Ag/AgCl electrochemical half-cells plug directly into the probes. Stray capacitance can be reduced by placing the included driven guard shield over the microelectrode holder at the end of the probe.
Capacity Compensation
Channel A can compensate up to 10 pF of electrode shunt capacity and Channel B can compensate up to 50 pF.
Tickler Circuit
Assists in cell penetration. The frequency and amplitude of the oscillations may be varied for differences in membrane thickness or cell size. The duration of tickle can be controlled either by using the momentary switch, a foot switch, or by applying a signal to the remote tickler input.
Active Filters
Low pass settings on a -40 dB/decade active filter vary the cutoff from 1 to 30 kHz. Either probe or bridge outputs may be selected for filtering.
Current Injection
Channel B can eject current through the microelectrode by applying a command signal to the stimulus input connector; the resulting output from the probe will then be a constant current replica of the input signal. Two ranges of current delivery are provided: 50 nA and 500 nA or by an external source. This source can be useful for delivering hyperpolarizing currents to stabilize the cell membrane potential and as a holding current for microiontophoresis.
Bridge Balance
Subtracts the excess electrode voltage associated with delivering current through the recording micropipette. Electrode resistances up to 1000 MΩ can be balanced in two ranges. The balanced signal is available from x10 or x50 front panel output connectors.
Independent Outputs
The Duo773 has an output for each probe independent of gain filtering or balancing. In addition the Duo773 has a 10x and a 50x output for easy integration to most data aquisition programs.
Specifications
| HEADSTAGE (PROBE) | 712P (red, port B), 715P (blue, port A) |
| ACTIVE PROBE INPUT IMPEDANCE 712P (red, port B) | >10^11ohm |
| ACTIVE PROBE INPUT IMPEDANCE 715P (blue, port A) | 10^15ohm |
| GAIN 712P (red, port B) | x1, x10 |
| GAIN 715P (blue, port A) | x1 |
| OUTPUT RESISTANCE 712P (red, port B) | 100ohm |
| OUTPUT RESISTANCE (blue, port A) | 100ohm |
| OUTPUT VOLTAGE RANGE 712P (red, port B) | ±10 V |
| OUTPUT VOLTAGE RANGE (blue, port A) | ± 10V |
| MAXIMUM INPUT VOLTAGE 712P (red, port B) | ±15V |
| MAXIMUM INPUT VOLTAGE (blue, port A) | ±15 V |
| PROBE LEAKAGE CURRENT 712P (red, port B) | 5 X 10-12 A |
| PROBE LEAKAGE CURRENT (blue, port A) | 10-14 A |
| DC POSITION ADJUST RANGE 712P (red, port B) | ± 300 mV |
| DC POSITION ADJUST RANGE (blue, port A) | ± 300 mV |
| ELECTRODE RESISTANCE TEST CURRENT 712P (red, port B) | 1nA |
| ELECTRODE RESISTANCE TEST CURRENT (blue, port A) | 1pA, 1nA selectable |
| INPUT CAPACITY COMPENSATION 712P (red, port B) | +10 to -50Pf |
| INPUT CAPACITY COMPENSATION (blue, port A) | 0 to -10pF |
| NOISE:Input shorted 712P (red, port B) | <50uV p-p 10kHz bandwidth |
| NOISE:Input shorted (blue, port A) | <50uV p-p 10kHz bandwidth |
| NOISE:20 MΩ carbon resistor 712P (red, port B) | <200uV p-p 10kHz bandwidth |
| NOISE:20 MΩ carbon resistor (blue, port A) | <200uV p-p 10kHz bandwidth |
| RISE TIME:10-90% direct input small signal | 1us, typical |
| RISE TIME:10-90% through 20MOhm (-C “on”) | 25us, typical |
| CURRENT INJECTION: Internal DC Current 712P (red, port B) | ± 50nA low range, ± 500nA high range |
| CURRENT INJECTION: Externally commanded Current 712P (red, port B) | ± 500nA low range, ±5uA high range |
| CURRENT INJECTION: External current command factor 712P (red, port B) | 20mV/nA low range, 2mV/nA high range |
| CURRENT INJECTION: Current monitor 712P (red, port B) | 100mV/nA low range, 10mV/nA high range |
| CURRENT INJECTION: Compliance 712P (red, port B) | 3V low range, 10V high range |
| CURRENT INJECTION: Bridge balance 712P (red, port B) | 0-100ohm, 0-1000ohm |
| CURRENT INJECTION: Bridge amplifier gain 712P (red, port “Bâ€) | x 10, x 50 |
| LOW PASS FILTER | 40 dB/decade, continuously variable 1-30 kHz |
| METER SECTION: Display | 3.5-digit LED |
| METER SECTION: Ranges | 200 mV, 2000 mV, 20 V, 200 nA, 2000 nA |
| METER SECTION: Accuracy and resolution | 1 digit |
| DIMENSIONS: Instrument | 17 x 5.25 x 10 in. (43 x 13 x 25 cm) |
| DIMENSIONS: Probe | Diameter: 12 mm Length: 34mm |
| POWER | 95-135V or 220-240V, 50/60Hz |
| SHIPPING WEIGHT | 15 lb (7 kg) |
| CERTIFICATION | CE, CSA |
